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mcf 7 human breast cancer cell line  (ATCC)


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    ATCC mcf 7 human breast cancer cell line
    Mcf 7 Human Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/breast+cancer+cell+line+mcf+7/MCF7/pm42821135-47-1-37
    Average 99 stars, based on 35046 article reviews
    mcf 7 human breast cancer cell line - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Thymoquinone decreases cell proliferation and immune evasion of breast cancer cells by reducing CD55 and CD114 levels.
    Article Snippet: Cell proliferation has emerged as a distinctive key feature needed for tumor development [1].. The continuous growth of tumor cells involves altered proliferation of the cell, one of the hallmarks of cancer [2].. Proliferation of cancer cells is mediated by aberrant expression of growth-related proteins, the membranebound receptors [3].

    Article Title: Actin-regulated plasticity as a key determinant of the bidirectional switch between chemoresistance and resensitization in triple-positive breast cancer.
    Article Snippet: Aim: Tamoxifen-induced chemoresistance promotes cancer cell survival, progression, and metastasis.. Therefore, we investigated and validated tamoxifen resistance involvement in cancer progression.. Methods: Tamoxifen resistance was induced in MCF7 cells; subsequently, fluorescence microscopy and proteomics were used to assess actin-mediated cellular alterations.

    Article Title: Single-cell transcriptomics identify mechanical-memory-associated cell states in metastatic HR + breast cancer.
    Article Snippet: .. Cell culture and In vitro mechanical memory experiment Cell culture: The HR+ breast cancer cell line MCF-7 and ZR-751 were obtained from ATCC, and cultured in Dulbecco’s Modifie ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS d Eagle Medium (DMEM, Corning) and RPMI-1640 Medium (164 0, Corning) correspondingly, supplemented with 10% FBS, 1% p enicillin–streptomycin, and 10 μg/ml bovine pancreas insulin (Sig ma-Aldrich). .. Cell culture and In vitro mechanical memory experiment Cell culture: The HR+ breast cancer cell line MCF-7 and ZR-751 were obtained from ATCC, and cultured in Dulbecco’s Modifie ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS d Eagle Medium (DMEM, Corning) and RPMI-1640 Medium (164 0, Corning) correspondingly, supplemented with 10% FBS, 1% p enicillin–streptomycin, and 10 μg/ml bovine pancreas insulin (Sig ma-Aldrich).

    Article Title: Sequence-Independent In Situ Imaging of GlycoRNA in Living Cells and Tissues based on COMPASS
    Article Snippet: .. Breast cancer cell line MCF-7 (HTB-22, ATCC) and non-tumorigenic breast cell line MCF-10A (CRL-10317, ATCC) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS, Tianhang), 100 μg/ml penicillin, and 100 μg/ml streptomycin. .. Hela cell line (CRM-CCL-2, ATCC), HepG2 cell line (HB-8065, ATCC), 4T1 cell line (CRL-2539, ATCC), LM3 cell line (SCSP-5093), and PC-12 cell line (CRL-1721, ATCC) were treated in DMEM supplemented with 10% FBS, 100 μg/ml penicillin, 100 μg/ml streptomycin, and 4.5g L -1 D-glucose.

    other:

    Article Title: Method of detecting cancer or cancer cells
    Article Snippet: The breast cancer cell line MCF-7, was obtained from the American Type Culture Collection.

    In Vitro:

    Article Title: Single-cell transcriptomics identify mechanical-memory-associated cell states in metastatic HR + breast cancer.
    Article Snippet: .. Cell culture and In vitro mechanical memory experiment Cell culture: The HR+ breast cancer cell line MCF-7 and ZR-751 were obtained from ATCC, and cultured in Dulbecco’s Modifie ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS d Eagle Medium (DMEM, Corning) and RPMI-1640 Medium (164 0, Corning) correspondingly, supplemented with 10% FBS, 1% p enicillin–streptomycin, and 10 μg/ml bovine pancreas insulin (Sig ma-Aldrich). .. Cell culture and In vitro mechanical memory experiment Cell culture: The HR+ breast cancer cell line MCF-7 and ZR-751 were obtained from ATCC, and cultured in Dulbecco’s Modifie ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS d Eagle Medium (DMEM, Corning) and RPMI-1640 Medium (164 0, Corning) correspondingly, supplemented with 10% FBS, 1% p enicillin–streptomycin, and 10 μg/ml bovine pancreas insulin (Sig ma-Aldrich).

    Stable Transfection:

    Article Title: Redox regulation of cell migration via Nischarin S-glutathionylation
    Article Snippet: .. Human embryonic kidney 293 (HEK293) cell line stably expressing GS M4 (HEK293-GS M4) [ ], breast cancer cell line MDA-MB-231 (ATCC, HTB-26), and breast cancer cell line MCF-7 (ATCC, HTB-22) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (Hyclone, Cytiva), penicillin (100 units/mL) and streptomycin (100 μg/mL) (Pen-Strep, Invitrogen) at 37°C in a 5 % CO 2 environment. .. Full-length Nischarin (Origene, pCMV6-myc-DDK-Nischarin, Cat# RC206026) was used to generate mutants by site-directed mutagenesis.

    Expressing:

    Article Title: Redox regulation of cell migration via Nischarin S-glutathionylation
    Article Snippet: .. Human embryonic kidney 293 (HEK293) cell line stably expressing GS M4 (HEK293-GS M4) [ ], breast cancer cell line MDA-MB-231 (ATCC, HTB-26), and breast cancer cell line MCF-7 (ATCC, HTB-22) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (Hyclone, Cytiva), penicillin (100 units/mL) and streptomycin (100 μg/mL) (Pen-Strep, Invitrogen) at 37°C in a 5 % CO 2 environment. .. Full-length Nischarin (Origene, pCMV6-myc-DDK-Nischarin, Cat# RC206026) was used to generate mutants by site-directed mutagenesis.

    Multiple Displacement Amplification:

    Article Title: Redox regulation of cell migration via Nischarin S-glutathionylation
    Article Snippet: .. Human embryonic kidney 293 (HEK293) cell line stably expressing GS M4 (HEK293-GS M4) [ ], breast cancer cell line MDA-MB-231 (ATCC, HTB-26), and breast cancer cell line MCF-7 (ATCC, HTB-22) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (Hyclone, Cytiva), penicillin (100 units/mL) and streptomycin (100 μg/mL) (Pen-Strep, Invitrogen) at 37°C in a 5 % CO 2 environment. .. Full-length Nischarin (Origene, pCMV6-myc-DDK-Nischarin, Cat# RC206026) was used to generate mutants by site-directed mutagenesis.

    Modification:

    Article Title: Redox regulation of cell migration via Nischarin S-glutathionylation
    Article Snippet: .. Human embryonic kidney 293 (HEK293) cell line stably expressing GS M4 (HEK293-GS M4) [ ], breast cancer cell line MDA-MB-231 (ATCC, HTB-26), and breast cancer cell line MCF-7 (ATCC, HTB-22) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (Hyclone, Cytiva), penicillin (100 units/mL) and streptomycin (100 μg/mL) (Pen-Strep, Invitrogen) at 37°C in a 5 % CO 2 environment. .. Full-length Nischarin (Origene, pCMV6-myc-DDK-Nischarin, Cat# RC206026) was used to generate mutants by site-directed mutagenesis.



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    Cytotoxic effect of compounds 5a-5e on MCF-7 cells following exposure to different concentrations (10, 20, 60, and 100 μM). Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the negative control group (untreated cells); doxorubicin was used as the positive control.

    Journal: Research in Pharmaceutical Sciences

    Article Title: Molecular docking, synthesis, and cytotoxic evaluation of novel quinazoline-quinazolinone hybrid compounds

    doi: 10.4103/RPS.RPS_150_24

    Figure Lengend Snippet: Cytotoxic effect of compounds 5a-5e on MCF-7 cells following exposure to different concentrations (10, 20, 60, and 100 μM). Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the negative control group (untreated cells); doxorubicin was used as the positive control.

    Article Snippet: The MCF-7 (human breast cancer) cell line was acquired from the Pasteur Institute of Iran (Tehran, Iran).

    Techniques: Comparison, Negative Control, Positive Control